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	Provedor de dados Electron. J. Biotechnol. (1) Autor Jiang,Keji (1) Ma,Lingbo (1) Pi,Yan (1) Zhang,Dan (1) Zhang,Fengying (1) Zhu,Houze (1) Mais... Palavra-chave Optic stalk (1) Potassium acetate (1) RNA isolation (1) Scylla paramamosain (1) Tipo do documento Journal article (1) Ano 2012 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Improved isolation of good-quality total RNA from the optic stalk of Mud crab, Scylla paramamosain Electron. J. Biotechnol. Zhu,Houze; Jiang,Keji; Zhang,Fengying; Zhang,Dan; Pi,Yan; Ma,Lingbo. An improved and efficient protocol was developed based on the TaKaRa RNAiso Plus Kit (Code: D9108A) for isolating good-quality total RNA from the optic stalk of mud crab, Scylla paramamosain. The protocol was based on the Trizol method with modifications. The carapace overlapping the optic stalk was retained with RNA in regular protocol. In order to remove the abundant deposition correlative with the carapace which makes the isolation of RNA particularly difficult, 5M potassium acetate solution (pH = 6.0) was added before the precipitation of RNA, and the temperature of RNA deposition was also decreased to -70ºC to ensure the stabilization of RNA. Good-quality total RNA from the optic stalk of S. paramamosain could be easily isolated with this modified... Tipo: Journal article Palavras-chave: Optic stalk; Potassium acetate; RNA isolation; Scylla paramamosain. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200005 Registros recuperados: 1 Primeira ... 1 ... Última

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